| MIDWEST REGION | WEST REGION | ||
| 1 | Kentucky | 1 | Wisconsin |
| 16 | Play-in Manhattan/N. Florida | 16 | Coastal Carolina |
| 8 | St. John’s | 8 | Xavier |
| 9 | Dayton | 9 | N.C. State |
| 5 | Louisville | 5 | SMU |
| 12 | Buffalo | 12 | Play-in: Boise State/BYU |
| 4 | Northern Iowa | 4 | North Carolina |
| 13 | Eastern Washington | 13 | Harvard |
| 3 | Notre Dame | 3 | Oklahoma |
| 14 | New Mexico State | 14 | Northeastern |
| 6 | Michigan State | 6 | San Diego State |
| 11 | Texas | 11 | LSU |
| 7 | VCU | 7 | Providence |
| 10 | Purdue | 10 | Stephen F. Austin |
| 2 | Kansas | 2 | Arizona |
| 15 | UAB | 15 | Texas Southern |
| EAST REGION | SOUTH REGION | ||
| 1 | Villanova | 1 | Duke |
| 16 | Lafayette | 16 | Play-in: Hampton/Robert Morris |
| 8 | Cincinnati | 8 | Iowa |
| 9 | Ohio State | 9 | Davidson |
| 5 | Arkansas | 5 | Wichita State |
| 12 | Wyoming | 12 | Wofford |
| 4 | West Virginia | 4 | Baylor |
| 13 | Valparaiso | 13 | Georgia State |
| 3 | Maryland | 3 | Iowa State |
| 14 | Albany | 14 | UC Irvine |
| 6 | Georgetown | 6 | Utah |
| 11 | Colorado State | 11 | Play-in: Indiana/Temple |
| 7 | Butler | 7 | Oregon |
| 10 | Georgia | 10 | Oklahoma State |
| 2 | Virginia | 2 | Gonzaga |
| 15 | Belmont | 15 | North Dakota State |
